ProteinChip®Array preparation in 3 steps:
Step 1: Application of crude biological Samples such as serum, urine, CSF and cell lysates on spot
Step 2: Removal of unbound proteins with washes
Step 3: Application of Energy absorbing molecules on each spot for desorption and ionization of proteins
The desorption and ionization of proteins bound to the chromatographic surface of the array is performed by a nitrogen laser. The ProteinChip Software calculates the mass of each protein and peptide detected. The mass/charge values and quantities of the proteins or peptides can be viewed in different formats, such as gel or line view.