LDI - Rules of Use

LDI Flow Cytometry Facility Rules of Use

1. The flow cytometry analysis room, E-417, is a Biosafety Level 1 (BSL1) Lab.Samples at or above BSL2 are not permitted to be run on the flow cytometers and cell counter (BD LSR Fortessa, BD FACSCanto II and Denovix CellDrop). If you wish to run primary human, virus-infected, or any other BSL2 samples on these instruments, they must be fixed!
2. The cell sorting room, E-417.1, is a BSL2 Lab. Users must submit the Cell Sorting Request form and, if applicable, disclose any risk factors by completing the High Risk Cell Sorting form at time of booking.
3. Acknowledge the LDI Flow Cytometry Facility in any publications (posters, presentations, papers,…) that includes data acquired at the facility and please send us the reference.
4. Before being issued online booking access and using LDI Flow Cytometry Facility instruments, ALL USERS MUST:

Be trained by an LDI Flow Cytometry Facility staff.
Users are not permitted to train other new users.
Duly complete and file in a User Information Form.
Provide account information for billing purposes.

5. Users can only use instruments on which they have been trained.
6. Training sessions are billed per hour and the user can bring a few samples similar to those with which they will be working on. This makes the training more specific to a given application and helps address specific sample issues.
7. Users will be charged for instrument login/logout time with a minimum charge of 15 minutes for each booking.
8. If a user did not cancel within 24 hours of their start time and does not show up for their reservation, they will be billed double for the entire booking.
9. Users must shutdown flow cytometers if:

10. If users do not show up during the first half-hour of their booking, the instrument is considered available for others to use. As per statement 8, the user at fault will be billed twice for the entire booking.
11. Users are required to use at least 75% of the booked time for reservations of less than 2 hours. As for reservations of more than 2 hour, a 30min overbooking is permitted. Repeated offenders will be billed double their booked time.
12. Users must clear all experiments from the FACS Diva software database before logging out. Experiments left on FACS Diva will be deleted without warning by facility staff at the minimum technical assistance rate ($65.00) per user.
13. When technical assistance is required, it will be by appointment only.
14. Users will NOT be charged if the equipment is not functioning. Please inform local managers or the facility director immediately if there is a problem with the equipment.
15. Users will have their privileges revoked temporarily or permanently for any misconduct, equipment misuse or non-standard manipulation; at the discretion of the LDI Flow Cytometry Facility staff.
16. Booking priority will be given in the following order:

17. The facility director or local managers reserve the right to make final decisions where there is a question of user priority.
18. The facility assumes no responsibility for data stored on the LDI Flow Cytometry Facility instruments and computers. Data left on the LDI Flow Cytometry Facility instruments and computers can be deleted without notice. NO CD-RW, DVD-RW or USB portable drives are allowed on any Windows OS acquisition computers as they can carry viruses infecting the computers.
19. No third party software, drivers nor plugins shall be installed on the core computer stations without prior approval of the facility manager.
20. Users who wish to take a FlowJo license on loan must first book the dongle online. There is a 3 consecutive day limit on each FlowJo dongle loan. All license dongles must be returned before 12:00 every Friday, or last day of the week. Weekend loans start Friday after 12:00. Your lab is responsible to provide a replacement in the event of a loss or stolen FlowJo dongle.

These rules are subject to change if deemed necessary by the LDI Flow Cytometry Facility staff.