Notre laboratoire de recherche a été créé en janvier 2014 à l’Institut Lady Davis de recherches médicales sur le chemin Ste-Catherine à Montréal. Nous faisons partie de l’Hôpital général juif de Montréal et de la division d’urologie du département de chirurgie de l’Université McGill.
Notre premier axe principal se concentre sur la physiologie et la pharmacologie des voies urinaires inférieures, en relation avec la maladie prostatique bénigne et le dysfonctionnement mictionnel. Notre équipe développe des projets de science translationnelle utilisant des techniques de laboratoire (culture cellulaire, CrisprCas9, microARN, microscopie confocale…) et des études cliniques sur des cohortes de patients atteints de ces pathologies.
Notre deuxième axe est centré sur le cancer de la prostate. En partenariat avec le chef du service d’urologie, nous utilisons l’immuno-histochimie CODEX et le SOMAscan pour analyser le profil inflammatoire de biopsies de la prostate de patients masculins avant et après traitement par thérapie focale. Ce projet vise à améliorer le pronostic du cancer de la prostate
La principale source de financement est l’Association des urologues du Canada, le Réseau québécois de recherche sur le vieillissement (RQRV) et la Fondation de l’HGJ.
From left to right: Chantal Autexier, Jian Qin, Adrian Young, Patrick Lambert-Lanteigne, Linda Pallotto, Alexandre Garus.
Au fil des ans, nous avons développé une expertise en science fondamentale et clinique, en mettant au point de nombreuses techniques différentes appliquées à l’étude de l’appareil urogénital.
La recherche en laboratoire impliquait la manipulation des animaux (enregistrements physiologiques et interventions chirurgicales) et des protocoles de laboratoire. Des modèles de dysfonctionnement mictionnel ont été développés dans les quartiers des animaux en utilisant des colonies de rats (Sprague-Dawley) et de souris (TallyHo, SWR, traitées à la streptozotocine). Les interventions chirurgicales comprennent l’insertion d’un cathéter en polyéthylène à l’intérieur de la vessie pour la cystométrie et l’obstruction partielle de l’urètre. La cystométrie fournit des informations sur les caractéristiques de la vessie in vivo pendant la miction et est associée à des bains d’organes qui permettent de mesurer les propriétés contractiles des bandelettes vésicales dans un environnement de contrôle (milieu de Krebs-Ringer sous saturation d’oxygène). Outre les protocoles couramment utilisés (immunoblotting, immunohistochimie confocale, qPCR…), nous avons développé des outils biomoléculaires, notamment l’isolement et la caractérisation des microARN, la délétion génomique par CrisprCas9 que nous avons réalisés à la fois sur des cultures de cellules primaires et in vivo. La recherche clinique translationnelle est réalisée à partir d’échantillons d’urine et de sang pour la métabolomique, la protéomique et la caractérisation des ARNm.
Notre deuxième domaine de recherche est centré sur le cancer de la prostate. Les patients du service d’urologie de l’Hôpital général juif subissent une thérapie focale pour traiter les cellules cancéreuses de la prostate. Les biopsies avant et après le traitement sont analysées par immuno-histochimie multiplex (CODEX) en utilisant des anticorps conjugués à des oligonucléotides pour mesurer jusqu’à 60 marqueurs et par SOMAscan pour analyser le profil protéomique des biopsies. L’analyse se concentre sur les cellules immunitaires, les cellules suppressives dérivées du myéloïde et les macrophages associés à la tumeur, des cellules qui favorisent le cancer de la vessie. Ces techniques permettent un meilleur pronostic après la thérapie.
Contemporary research has uncovered a role for environmental copper in the development and progression of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Previous research implicated non-ceruloplasmin bound copper in the aggravation of neurodegenerative phenotypes. Our research explores the role of copper in ALS progression through monitoring of copper biomarkers and analyses of copper metabolism gene expression.
Lorem ipsum dolor sit amet consectetur adipisicing elit. Maxime mollitia, molestiae quas vel sint commodi repudiandae consequuntur voluptatum laborum numquam blanditiis harum quisquam eius sed odit fugiat iusto fuga praesentium optio, eaque rerum! Provident similique accusantium nemo autem. Veritatis obcaecati tenetur iure eius earum ut molestias architecto voluptate aliquam nihil, eveniet aliquid culpa officia aut! Impedit sit sunt quaerat, odit, tenetur error, harum nesciunt ipsum debitis quas aliquid. Reprehenderit, quia. Quo neque error repudiandae fuga? Ipsa laudantium molestias eos sapiente officiis modi at sunt excepturi expedita sint? Sed quibusdam recusandae alias error harum maxime adipisci amet laborum. Perspiciatis minima nesciunt dolorem! Officiis iure rerum voluptates a cumque velit quibusdam sed amet tempora. Sit laborum ab, eius fugit doloribus tenetur fugiat, temporibus enim commodi iusto libero magni deleniti quod quam consequuntur!
I obtained my BSc in pharmacology at University of Sherbrooke and graduated from University of Montreal with a MSc in pharmaceutical sciences in Prof. Huy Ong’s laboratory developing a ligand screening method for the CD36 receptor using SPR (surface plasmon resonance) biosensor. I then worked for Prof. Arnim Pause at McGill University trying to elucidate the interaction pattern between the folliculin protein with its binding partner AMPK. I then joined Prof. Chantal Autexier’s laboratory as a research assistant where I work in telomere biology, manage the lab and mentor undergraduate and graduate students. I helped characterize the binding pocket of two telomerase domain/motifs, IFD and TEN, with its binding partner TPP1, and I now focus on the telomerase-independent alternative lengthening of telomere mechanism.
I received a BSc in Molecular Biology & Genetics from McMaster University in 2017. I am currently studying how mutations of dyskerin, a telomerase-associated protein, affect its interaction with other proteins, subcellular localization, and telomerase RNA abundance.
I am a Belizean pursuing a PhD in Anatomy and Cell biology at McGill University in Montreal. I completed my BSc in Biochemistry and Cellular and Molecular Biology and my MSc in Biochemistry at Memorial University in Newfoundland Canada. My research interest involves increased understanding stem and cancer cell dynamics and redox biology to be applied to therapeutics for health span extension.
I come from Brazil, where I graduated in Biochemical Engineering at the Federal University of Paraná. I have more than four years of professional experience in manufacturing and quality assurance of reagents for molecular diagnostics. Now I’m interested in better understanding the role of the protein dyskerin and H/ACA ribonucleoprotein complexes in regulating telomere biology and cellular aging.
Linda completed her BSc in Biology at the University of Waterloo. During her undergraduate degree, she worked as a co-op student in the lab of Dr. Paul Mains where her project used C. elegans to describe gene variants that cause drug resistance in parasitic nematodes. After graduating, she worked for a year in the lab of Dr. Ryan Yuen at SickKids in Toronto investigating short tandem repeat expansions using Nanopore and Sanger sequencing. She is now pursuing her MSc in the Autexier lab and is interested in how a region of telomerase, known as the insertions in fingers domain (IFD), is involved in its localization and activity at the telomeres. When not in lab, Linda enjoys listening to music or watching movies while she knits.
Madelyn is a PhD student in the department of Experimental Medicine. She uses mouse modelling to study Diffuse Large B Cell Lymphoma. In particular, she has found that mutations in the transcription factor STAT6 are enriched at disease relapse, and I study how this contributes to remodeling of the tumour microenvironment.
Rowa is a PhD candidate in the department of Experimental Medicine. She completed her BSc in Microbiology and Immunology in Saudi Arabia, then completed two MScs in Genetics and Parasitology at McGill University. Her work investigates tungsten toxicity focusing on SLC2A2 as a transporter and the effects on mature B lymphocytes.
Vincenza is an MSc. Student in the Department of Pharmacology. She completed her BSc. in Pharmacology at McGill University. Her project aims to elucidate the role of e-cigarette use on the murine cardiopulmonary system. Specifically, she is focusing on metal deposition in the lungs, and the progression of atherosclerosis due to these exposures.
Nazli Zengin is an MSc. student in the Department of Pharmacology. She has a background in pharmacology and environment. Her work currently focuses on elucidating how sex and diet modulate arsenic toxicity and its immunological and cardiovascular consequences.
Andrew is an MSc. student in the Department of Pharmacology who graduated from the B.Sc. program in Biochemistry at McGill in 2021. He works on a proteomics project centred around Arsenic 3-Methyltransferase and the question about whether it serves an additional function.
Raymond is an MSc. Student in the Department of Pharmacology who completed his BSc. in Environmental Health Sciences from the UNC Chapel Hill as a as a Morehead-Cain Scholar. He received the McCall MacBain Scholarship in 2021. His project studies the mechanism of tungsten on mTORC1, a major regulator of cell growth and proliferation.
Nikola is an incoming MSc Student who completed his undergraduate degree in
Microbiology and Immunology at McGill University. He is currently working on an NSERC
summer project that assesses the functional characterization of macrophages exposed to arsenic using murine bone marrow-derived macrophages.
Roni is a second-year BSc student in Chemistry at McGill. As an undergraduate student, she works on various projects across the lab, but is currently focused elucidating the correlation between nonalcoholic fatty liver disease and vaping.
Contemporary research has uncovered a role for environmental copper in the development and progression of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Previous research implicated non-ceruloplasmin bound copper in the aggravation of neurodegenerative phenotypes. Our research explores the role of copper in ALS progression through monitoring of copper biomarkers and analyses of copper metabolism gene expression.
Lorem ipsum dolor sit amet consectetur adipisicing elit. Maxime mollitia, molestiae quas vel sint commodi repudiandae consequuntur voluptatum laborum numquam blanditiis harum quisquam eius sed odit fugiat iusto fuga praesentium optio, eaque rerum! Provident similique accusantium nemo autem. Veritatis obcaecati tenetur iure eius earum ut molestias architecto voluptate aliquam nihil, eveniet aliquid culpa officia aut! Impedit sit sunt quaerat, odit, tenetur error, harum nesciunt ipsum debitis quas aliquid. Reprehenderit, quia. Quo neque error repudiandae fuga? Ipsa laudantium molestias eos sapiente officiis modi at sunt excepturi expedita sint? Sed quibusdam recusandae alias error harum maxime adipisci amet laborum. Perspiciatis minima nesciunt dolorem! Officiis iure rerum voluptates a cumque velit quibusdam sed amet tempora. Sit laborum ab, eius fugit doloribus tenetur fugiat, temporibus enim commodi iusto libero magni deleniti quod quam consequuntur!
Notre laboratoire possède l’équipement pour le processus d’histologie et d’histochimie et a accès au Laboratoire d’imagerie cellulaire de l’ILD sous la supervision de Marie-Lyne Fillion (microscope confocal et LSM800 Airyscan). La RT-qPCR pour la quantification de l’ARN et des microARN est réalisée au Centre du cancer Segal et nous avons développé une expertise CrisprCas9 pour l’édition génomique. Des techniques plus classiques sont également présentes dans notre laboratoire, immunoblotting, RTPCR, cultures de cellules primaires… Nous possédons deux bains d’organes permettant de mesurer la contractilité de la bandelette vésicale (2 machines au total). Nous avons développé plusieurs modèles de dysfonctionnement de la vessie chez le rat (obstruction partielle de la vessie) et la souris (diabète de type 1 induit par la streptozotocine, TallyHo) et les quartiers animaliers de l’ILD apportent leur soutien et leur expertise en matière de manipulation et de chirurgie des animaux.
Pour la recherche sur le cancer de la prostate, nous avons accès à l’appareil d’immuno-histochimie CODEX et au SOMAscan pour une analyse protéomique sensible au sein de l’ILD.
Contemporary research has uncovered a role for environmental copper in the development and progression of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Previous research implicated non-ceruloplasmin bound copper in the aggravation of neurodegenerative phenotypes. Our research explores the role of copper in ALS progression through monitoring of copper biomarkers and analyses of copper metabolism gene expression.
Lorem ipsum dolor sit amet consectetur adipisicing elit. Maxime mollitia, molestiae quas vel sint commodi repudiandae consequuntur voluptatum laborum numquam blanditiis harum quisquam eius sed odit fugiat iusto fuga praesentium optio, eaque rerum! Provident similique accusantium nemo autem. Veritatis obcaecati tenetur iure eius earum ut molestias architecto voluptate aliquam nihil, eveniet aliquid culpa officia aut! Impedit sit sunt quaerat, odit, tenetur error, harum nesciunt ipsum debitis quas aliquid. Reprehenderit, quia. Quo neque error repudiandae fuga? Ipsa laudantium molestias eos sapiente officiis modi at sunt excepturi expedita sint? Sed quibusdam recusandae alias error harum maxime adipisci amet laborum. Perspiciatis minima nesciunt dolorem! Officiis iure rerum voluptates a cumque velit quibusdam sed amet tempora. Sit laborum ab, eius fugit doloribus tenetur fugiat, temporibus enim commodi iusto libero magni deleniti quod quam consequuntur!
2023
2022
2021
2020
2019
2018
2017
2016
2015
2014
Uri Saragovi, Université McGill, Montréal, Canada
Indra Gupta, Université McGill, Montréal, Canada
Jeremy Burton, Western University, London, Canada
Stephane Bolduc, Université Laval, Québec, Canada
Lori Lerner, Boston University School of Medicine, Boston, USA
Gilles Karsenty, Aix-Marseille Université, Marseille, France
Dr. Brandon Pearson, Columbia University, New York
Dr. John Wise, University of Louisville, Kentucky
Dr. Wilson Miller, Lady Davis Institute, Montreal
Dr. Sonia del Rincon, Lady Davis Institute, Montreal
Dr. Carolyn Baglole, RI-MUHC, McGill University, Montreal
Dr. Jun Ding, RI-MUHC, McGill University, Montreal
Dr. Susan Gaskin, Dep’t of Chemical Engineering, McGill University, Montreal
Dr. Bernard Robaire, Dep’t of Pharmacology McGill University, Montreal
Dr. Brandon Pearson, Columbia University, New York
Dr. John Wise, University of Louisville, Kentucky
Lorem ipsum dolor sit amet consectetur adipisicing elit. Maxime mollitia, molestiae quas vel sint commodi repudiandae consequuntur voluptatum laborum numquam blanditiis harum quisquam eius sed odit fugiat iusto fuga praesentium optio, eaque rerum! Provident similique accusantium nemo autem. Veritatis obcaecati tenetur iure eius earum ut molestias architecto voluptate aliquam nihil, eveniet aliquid culpa officia aut! Impedit sit sunt quaerat, odit, tenetur error, harum nesciunt ipsum debitis quas aliquid. Reprehenderit, quia. Quo neque error repudiandae fuga? Ipsa laudantium molestias eos sapiente officiis modi at sunt excepturi expedita sint? Sed quibusdam recusandae alias error harum maxime adipisci amet laborum. Perspiciatis minima nesciunt dolorem! Officiis iure rerum voluptates a cumque velit quibusdam sed amet tempora. Sit laborum ab, eius fugit doloribus tenetur fugiat, temporibus enim commodi iusto libero magni deleniti quod quam consequuntur!
2023
Prix Kathryn Rolph : en reconnaissance de ses réalisations exceptionnelles et de son leadership, contribuant à l’avancement du département de chirurgie de la faculté de médecine de l’Université McGill.
2019
Jian Qin
Deanna MacNeil
CIHR CGS-M, CIHR Doctoral Award, Cole Foundation Studentship
Tsz Wai (Josephine) Chu
Johanna Mancini
FRQS doctoral award, Cole Foundation Studentship
Yasmin D’Souza
May Shawi
CIHR Doctoral Award, Cole Foundation Studentship
Marie Eve Brault
Johans Fakhoury
McGill University Faculty of Medicine Internal Studentship
Maria Antonietta Cerone
Tara Moriarty
CIHR Doctoral Award
Sylvain Huard
François Bachand
CIHR Doctoral Award
2022
Meilleur ePoster au 42ème Congrès de la Société internationale d’urolgie 9-13 novembre 2022
2022
2022
Meilleur résumé de science fondamentale – Society of Urodynamics, Female Pelvic Medicine and Urogenital Reconstruction Annual meeting Annual Meeting 2022
2021
Meilleur résumé dans la catégorie Science translationnelle lors du 6th Annual Multidisciplinary Benign Urology Research Symposium 29 avril 2021
2020
2018
Spinal Cord Best Review Paper Award pour les articles publiés en 2017, Spinal Cord (Nature Journal)
2018
2017
Réunion annuelle de la société internationale de la continence 2017 : meilleur résumé dans sa catégorie
2017
Johans Fakhoury
McGill University Faculty of Medicine Internal Studentship
Hannah Chui
NSERC USRA recipientCIHR CGS-M Award
Dominique Rinfret
Michelle Shen
NSERC USRA recipient
Ricky Kwan
McGill University Faculty of Medicine Internal Studentship
Yasmin D’Souza
May Shawi
CIHR Doctoral Award, Cole Foundation Studentship
Marie Eve Brault
Johans Fakhoury
McGill University Faculty of Medicine Internal Studentship
A few interesting links related to Chantal Autexier, her laboratory and herresearch themes:
Dr. Wilson Miller, Lady Davis Institute, Montreal
Dr. Sonia del Rincon, Lady Davis Institute, Montreal
Dr. Carolyn Baglole, RI-MUHC, McGill University, Montreal
Dr. Jun Ding, RI-MUHC, McGill University, Montreal
Dr. Susan Gaskin, Dep’t of Chemical Engineering, McGill University, Montreal
Dr. Bernard Robaire, Dep’t of Pharmacology McGill University, Montreal
Dr. Brandon Pearson, Columbia University, New York
Dr. John Wise, University of Louisville, Kentucky
Lorem ipsum dolor sit amet consectetur adipisicing elit. Maxime mollitia, molestiae quas vel sint commodi repudiandae consequuntur voluptatum laborum numquam blanditiis harum quisquam eius sed odit fugiat iusto fuga praesentium optio, eaque rerum! Provident similique accusantium nemo autem. Veritatis obcaecati tenetur iure eius earum ut molestias architecto voluptate aliquam nihil, eveniet aliquid culpa officia aut! Impedit sit sunt quaerat, odit, tenetur error, harum nesciunt ipsum debitis quas aliquid. Reprehenderit, quia. Quo neque error repudiandae fuga? Ipsa laudantium molestias eos sapiente officiis modi at sunt excepturi expedita sint? Sed quibusdam recusandae alias error harum maxime adipisci amet laborum. Perspiciatis minima nesciunt dolorem! Officiis iure rerum voluptates a cumque velit quibusdam sed amet tempora. Sit laborum ab, eius fugit doloribus tenetur fugiat, temporibus enim commodi iusto libero magni deleniti quod quam consequuntur!
Dr. Wilson Miller, Lady Davis Institute, Montreal
Dr. Sonia del Rincon, Lady Davis Institute, Montreal
Dr. Carolyn Baglole, RI-MUHC, McGill University, Montreal
Dr. Jun Ding, RI-MUHC, McGill University, Montreal
Dr. Susan Gaskin, Dep’t of Chemical Engineering, McGill University, Montreal
Dr. Bernard Robaire, Dep’t of Pharmacology McGill University, Montreal
Dr. Brandon Pearson, Columbia University, New York
Dr. John Wise, University of Louisville, Kentucky
Notre recherche a été soutenue par une subvention du Fonds de Recherche Québec Santé, de la Fondation des bourses de l’Association des urologues du Canada, du Réseau québécois de recherche sur le vieillissement (RQRV) et par des bourses d’études de l’Institut Lady Davis de recherches médicales, des IRSC et du FRQS.
La recherche sur le cancer de la prostate est soutenue par la Fondation de l’HGJ.
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