What is it?
The Zeiss LSM800 is an inverted laser-scanning microscope equipped with Airyscan,
allowing for super-resolution imaging. The Experiment Designer feature allows
for multidimensional acquisition of data with individual settings for each
component of the experiment. The tiling and positioning feature can create a
low magnification image preview for easier locating of samples at higher
magnifications, as well as use reference points to retain focus on large tiles
of uneven samples. Definite Focus is also present to prevent focus drift and
keep samples in focus.
Where is it?
Pavilion E, Room-412
Objective
Plan Plan-Neofluar / 10x, Air / 0.30 NA
Plan-Apochromat / 20x, Air / 0.80 NA
Plan-Apochromat / 40x, Oil / 1.40 NA
Plan-Apochromat / 63x, Oil / 1.40 NA
Detectors:
Three GaAsP PMTs, one of which is configured for Airyscan
Laser/ Filter
Four lasers at 405nm, 488nm, 561nm, and 640nm are present, with a variable secondary dichroic allowing for imaging of any emission wavelength between 410-700nm.
What is it?
The Quorum
Wave FX SD confocal microscope is a laser-based system using spinning disk
technology, which significantly reduces photobleaching and phototoxicity. This inverted
microscope is ideal for studying live cell activity in a chamber or fixed
samples with dim signals. Environmental control for live cell imaging via a
stage-top incubator for temperature, CO2, and humidity control is present on
this system.
Where is it?
Pavilion E, Room-512
Objective
HC PL APO / 10X, Air / 0.25 NA
HC PL APO / 20X, Air / 0.70 NA
HCX PL APO / 40X, Oil / 0.75-1.25 NA CS
HCX PL APO / 63X, Oil / 0.60-1.40 NA BL
Camera:
Hamamatsu EM-CCD Digital Camera
Laser/ Filter/ Common Fluorochromes/Dyes
What is it?
The Leica DM LB2 is a widefield microscope with an
upright stand. Light sources of 100W halogen lamps and 100W mercury lamps are
able to perform both bright-filed and fluorescence imaging on a slide. This
system is equipped with a monochrome and colour camera and is ideal for basic
fluorescence imaging and immunohistochemistry studies.
Where is it?
Pavilion E, Room-412
Objective
N Plan / 10X, Air/ 0.25 NA
HC PL Fluotar / 20X, Air/ 0.50 NA
HCX PL Fluotar / 40X, Air/ 0.75 NA/ Ph2
N Plan / 100X, Oil/ 1.25 NA
Camera:
Leica DFC 480 (Color Digital Camera)
Leica DFC 350 Fx (Monochrome Digital Camera)
What is it?
The Zeiss Axiovert 40 is a widefield inverted
microscope with a 100W halogen and mercury lamp. This system is ideal for
observation of live cells expressing endogenous fluorescent signals.
Where is it?
Pavilion E, Room-412
Lenses
CP Achromat/ 10X, Air/ 0.25 NA/ Ph1
LD A-Plan / 20X, Air/ 0.3 NA/ Ph1
LD A-Plan/ 40X, Air/ 0.5 NA/ Ph2
Camera:
No Camera is available