Zeiss LSM800 Confocal Microscope with Airyscan
 
What is it?
The Zeiss LSM800 is an inverted laser-scanning microscope equipped with Airyscan, allowing for super-resolution imaging. The Experiment Designer feature allows for multidimensional acquisition of data with individual settings for each component of the experiment. The tiling and positioning feature can create a low magnification image preview for easier locating of samples at higher magnifications, as well as use reference points to retain focus on large tiles of uneven samples. Definite Focus is also present to prevent focus drift during long-term acquisitions.
 
Where is it?
Pavilion E, Room-412

Objective
Plan Plan-Neofluar / 10x, Air / 0.30 NA
Plan-Apochromat / 20x, Air / 0.80 NA
Plan-Apochromat / 40x, Oil / 1.40 NA
Plan-Apochromat / 63x, Oil / 1.40 NA

Detectors:
Three GaAsP PMTs, one of which is configured for Airyscan

Laser/ Filter
Four lasers at 405nm, 488nm, 561nm, and 640nm are present, with a variable secondary dichroic allowing for imaging of any emission wavelength between 410-700nm.
 
 
Quorum Wave FX SD confocal microscope
What is it?
The Quorum Wave FX SD confocal microscope is a laser-based system using spinning disk technology, which significantly reduces photobleaching and phototoxicity. This inverted microscope is ideal for studying live cell activity in a chamber or fixed samples with dim signals. Environmental control for live cell imaging via a stage-top incubator for temperature, CO2, and humidity control is present on this system.

Where is it?
Pavilion E, Room-512

Objective
HC PL APO / 10X, Air / 0.25 NA
HC PL APO / 20X, Air / 0.70 NA
HCX PL APO / 40X, Oil / 0.75-1.25 NA CS
HCX PL APO / 63X, Oil / 0.60-1.40 NA BL

Camera:
Hamamatsu EM-CCD Digital Camera
 
Laser/ Filter/ Common Fluorochromes/Dyes 
 
 
 
Zeiss LSM 5 Pascal Confocal Microscope
 
What is it?
Zeiss LSM Pascal 5 is an inverted laser scanning microscope with fully motorized scanning modules. This system is ideal for quantitative measurements, such as colocalization study on fixed slides.

Where is it?
Pavilion E, Room-412

Objective
Plan Neofluar/ 10X, Air/ 0.40 NA
Plan Neofluar/ 40X, Air/ 0.75 NA
Plan Neofluar/ 63X, Oil/ 1.40 NA

Detector:
PMT
 
Laser/ Filter/ Common Fluorochromes/Dyes 
 
 
 
Leica DM LB2 Widefield Upright Microscope
 
What is it?
Leica DM LB2 is a upright widefield microscope. Light source of 100W halogen lamps and 100W mercury lamps enable to perform both bright-filed and fluorescence imaging on a slide. This system is equipped with a monochrome and a color camera and is ideal for basic epifluorescence imaging and immunohistochemistry studies.

Where is it?
Pavilion E, Room-412

Objective
N Plan / 10X, Air/ 0.25 NA
HC PL Fluotar / 20X, Air/ 0.50 NA
HCX PL Fluotar / 40X, Air/ 0.75 NA/ Ph2
N Plan / 100X, Oil/ 1.25 NA

Camera:
Leica DFC 480 (Color Digital Camera)
Leica DFC 350 Fx (Monochrome Digital Camera)
 
 
Zeiss Axiovert 40 CFL Widefield Inverted Microscope
 
What is it?
Zeiss Axiovert 40 is a widefield inverted microscope with 100W halogen and mercury lamp. This system is ideal for observation of live cell expressing endogenous fluoresce signal.

Where is it?
Pavilion E, Room-412

Lenses
CP Achromat/ 10X, Air/ 0.25 NA/ Ph1
LD A-Plan / 20X, Air/ 0.3 NA/ Ph1
LD A-Plan/ 40X, Air/ 0.5 NA/ Ph2

Camera:
No Camera is available
 
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